| 產(chǎn)品編號 | bs-0988R-AP |
| 英文名稱(chēng) | Rabbit Anti-CD71/Transferrin receptor/AP Conjugated antibody |
| 中文名稱(chēng) | 堿性磷酸酶（AP）標記的轉鐵蛋白受體抗體 |
| 別 名 | CD 71; CD71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TFRC; TR; Transferrin receptor (p90 CD71); Transferrin Receptor Protein 1; OTTHUMP00000208523; OTTHUMP00000208524; OTTHUMP00000208525; Transferrin receptor protein 1, serum form; TRFR. |
| 規格價(jià)格 | 100ul/2980元 購買(mǎi) 大包裝/詢(xún)價(jià) |
| 說(shuō) 明 書(shū) | 100ul |
| 研究領(lǐng)域 | 腫瘤 心血管 細胞生物 免疫學(xué) 信號轉導 干細胞 轉錄調節因子 細胞表面分子 細胞類(lèi)型標志物 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應 | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Horse, Guinea Pig, ) |
| 產(chǎn)品應用 | WB=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 74/85kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Transferrin receptor |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro. Function: Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Subunit: Homodimer; disulfide-linked. Binds one transferrin molecule per subunit. Interacts with SH3BP4. Subcellular Location: Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Post-translational modifications: N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. Similarity: Belongs to the peptidase M28 family. M28B subfamily. Contains 1 PA (protease associated) domain. Database links: Entrez Gene: 7037 Human Entrez Gene: 22042 Mouse Omim: 190010 Human SwissProt: P02786 Human SwissProt: Q62351 Mouse Unigene: 529618 Human Unigene: 28683 Mouse Unigene: 98672 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 轉鐵蛋白及轉鐵蛋白受體(TfnrR)是機體鐵代謝中的重要因子，鐵是細胞生長(cháng)增殖、代謝的必需元素,轉鐵蛋白受體(TfR)介導的胞吞作用是細胞攝取鐵的主要途徑。腫瘤細胞因增殖生長(cháng)旺盛,對鐵的需求高,所以腫瘤細胞有比正常細胞更多的轉鐵蛋白受體的表達。 |
